RESEARCH ARTICLE


In Vitro Elution Characteristics of Gentamicin and Vancomycin from Synthetic Bone Graft Substitutes



Gerrit Steffen Maier*, 1, Klaus Edgar Roth1, Stefan Andereya2, Klaus Birnbaum3, Christopher Niedhart4, Markus Lühmann5, Jörg Ohnsorge5, Uwe Maus6
1 Department of Orthopedic Surgery, Johannes-Guttenberg-University, Langenbeckstrasse 1, D-55131 Mainz, Germany
2 Orthopädische Gemeinschaftspraxis, Adalbertsteinweg 12, D-52070 Aachen, Germany
3 Orthopaedic Clinic Hennef, Adenauerplatz 1, D-53773 Hennef, Germany
4 Orthopädische Gemeinschaftspraxis, Liecker Str. 23, D-52525 Heinsberg, Germany
5 Orthopedic Center Oldenburger Münsterland, St. Antonius-Stift Emstek, Antoniusstr. 28, D-49685 Emstek, Germany
6 Department of Orthopedics and Special Orthopaedic Surgery, University Hospital for Orthopedic Surgery and Traumatology, Pius-Hospital, Georgstraße 12, D-26121 Oldenburg, Germany


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© Maier et al.; Licensee Bentham Open.

open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.

* Address correspondence to this author at the Department of Orthopedic Surgery, Johannes-Guttenberg-University, Langenbeckstrasse 1, D-55131 Mainz, Germany; Tel: 0049-6131-177302; Fax: 0040-6131-173416; E-mail: gerrit.s.maier@gmx.de


Abstract

Objects:

Beta tricalciumphosphate pellets loaded with individualized antibiotics may represent novel options in the treatment of osteomyelitis and infectious bone disease. Here, the in vitro antibiotic elution of vancomycin and gentamicin from the synthetic bone graft substitutes Cerasorb® and Cerasorb M® was tested.

Methods:

Antibiotic elution and concentration of gentamcin and vancomycin were measured using photometrically-based measurement and homogeneous particle-enhanced turbidimetric inhibition immunoassays (PETINIA).

Results:

Initially both materials showed a high release of the loaded antibiotics, with Cerasorb M® showing lower release levels for gentamicin and vancomycin than Cerasorb®. Gentamicin concentrations of Cerasorb M granules and Cerasorb were below the minimum detectiontreshold until day four and six of the experiment respectively. The vancomycin release-level followed a similar pattern, although the vancomycin concentration eluted by Cerasorb M® granules stayed above the detection threshold during the experimental time.

Conclusions:

Cerasorb® and Cersorb M® may represent a new treatment option in osteomyelitis and infectious bone disease.

Keywords: Cerasorb®, Cerasorb M®, Osteomyelitis, beta tricalciumphosphate.