Freezing of Rat Tibiae at -20°C Does Not Affect the Mechanical Properties of Intramedullary Bone/Implant-Interface: Brief Report

Michael Diefenbeck*, 1, Thomas Mückley1, Sergiy Zankovych2, Jörg Bossert2, Klaus D Jandt2, Christian Schrader3, Jürgen Schmidt3, Ulrich Finger4, Mathilde Faucon4
1 Klinik für Unfall-, Hand- und Wiederherstellungschirurgie, Universitätsklinikum Jena, Erlanger Allee 101, D-07747 Jena, Germany
2 Institute of Materials Science and Technology (IMT), Friedrich-Schiller-Universität Jena, Löbdergraben 32, D-07743 Jena, Germany
3 Innovent Technology Development, Prüssingstraße 27 B, D-07745 Jena, Germany
4 Königsee Implantate GmbH, OT Aschau - Am Sand 4, D-07426 Allendorf, Germany

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© Diefenbeck et al.; Licensee Bentham Open.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.

* Address correspondence to this author at the Klinik für Unfall-, Hand- und Wiederherstellungschirurgie, Universitätsklinikum Jena, Erlanger Allee 101, 07747 Jena, Germany; Tel: 0049 3641 9 322800; Fax: 0049 3641 9 322802; E-mail:



The effects of freezing-thawing cycles on intramedullary bone-implant interfaces have been studied in a rat model in mechanical pull-out tests.


Twenty TiAl6V4 rods (Ø 0.8 mm, length 10 mm) implanted in rat tibiae


10 rats underwent bilateral tibial implantation of titanium rods. At eight weeks, the animals were sacrificed and tibiae harvested for biomechanical testing. Eight tibiae were frozen and stored at -20°C for 14 days, the remaining eight were evaluated immediately post-harvest. Pull-out tests were used to determine maximum force and interfacial shear strength.


There were no significant differences between fresh and those of the frozen-thawed group in maximum force or in interfacial shear strength.


Frozen Storage of rat tibiae containing implants at -20° C has no effects on the biomechanical properties of Bone/ Implant interface.

Keywords: Bone, implant, bone-implant-interface, fixation, freezing/thawing cycles, storage.